頭蛋白(NOG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Noggin (NOG)
SYM1; SYNS1; Synostoses(Multiple)Syndrome 1; Symphalangism 1(Proximal)
- 編號KSC130Hu01
- 物種Homo sapiens (Human，人)相同的名稱，不同的物種。
- 試劑內(nèi)容包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍1.56-100ng/mL
- 可適靈敏度0.66ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載英文說明書 中文說明書
- 規(guī)格96T*596T*1096T*2096T*5096T*100
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對頭蛋白(NOG)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APC130Hu01 | 頭蛋白(NOG)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPC130Hu01 | 頭蛋白(NOG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC130Hu02 | 頭蛋白(NOG)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC130Hu61 | 頭蛋白(NOG)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC130Hu01 | 頭蛋白(NOG)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC130Hu02 | 頭蛋白(NOG)多克隆抗體 | WB; IHC; ICC; IP. |
| LAC130Hu71 | 頭蛋白(NOG)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAC130Hu81 | 頭蛋白(NOG)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAC130Hu21 | 頭蛋白(NOG)單克隆抗體 | WB |
| MAC130Hu22 | 頭蛋白(NOG)單克隆抗體 | WB; IHC |
| MAC130Hu24 | 頭蛋白(NOG)單克隆抗體 | WB; IHC |
| MAC130Hu26 | 頭蛋白(NOG)單克隆抗體 | WB; IHC |
| FAC130Hu02 | 抗頭蛋白(NOG)單克隆抗體 | FCM |
| LAC130Hu76 | 頭蛋白(NOG)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEC130Hu | 頭蛋白(NOG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC130Hu | 頭蛋白(NOG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSC130Hu01 | 頭蛋白(NOG)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSC130Hu01 | 頭蛋白(NOG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Arthritis Rheumatol | Imbalance between BMP2 and Noggin induces abnormal osteogenic differentiation of mesenchymal stem cells in ankylosing spondylitis[PubMed: 26413886] |
| Arthritis Rheumatol.? | Imbalance Between Bone Morphogenetic Protein 2 and Noggin Induces Abnormal Osteogenic Differentiation of Mesenchymal Stem Cells in Ankylosing Spondylitis[Pubmed:26413886] |
| Clin Exp Pharmacol Physiol. | Oxygen-glucose deprivation preconditioning protects neurons against oxygen-glucose deprivation/reperfusion induced injury via bone morphogenetic protein-7 mediated ERK, p38 and Smad signalling pathways.[Pubmed:26385023] |
| European cells & materials | The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells.[pubmed:28266688] |
| Arthritis?&?Rheumatology | mbalance?Between?Bone?Morphogenetic?Protein?2?and?Noggin?Induces?Abnormal?OsteogenicDifferentiation?of?Mesenchymal?Stem Cells in Ankylosing Spondylitis.[pubmed:26413886] |
| Biotechnology and?Applied?Biochemistry | The BMP antagonist Noggin is produced by osteoblasts in response to the presence of prostate cancer cells.[pubmed:28981962] |
| Heliyon | Efficient in vitro delivery of Noggin siRNA enhances osteoblastogenesis[pubmed:29167826] |
| Journal of Cellular Biochemistry | Low‐intensity pulsed ultrasound promotes bone morphogenic protein 9‐induced osteogenesis and suppresses inhibitory effects of inflammatory cytokines on cellular?…[Pubmed: 31006911] |
| Aging Male | Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment[Pubmed: 32160825] |
| J Reconstr Microsurg | Effect of Simvastatin Use in Free Tissue Transfer: An Experimental Study in a Rat Epigastric Free Flap Model[Pubmed: 31995818] |
| Biochem Biophys Rep | Slow diffusion on the monolayer culture enhances auto/paracrine effects of Noggin in differentiation of human iPS cells induced by BMP[Pubmed:35005256] |