心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)
Mini Samples ELISA Kit for Atrial Natriuretic Peptide (ANP)
ANH; ANF; CDD; Atrial Natriuretic Factor; Atrial Natriuretic Hormone; Atriopeptin; Cardionatrine; Cardiodilatin
- 編號MEA225Mu
- 物種Mus musculus (Mouse，小鼠)相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長3h
- 檢測范圍12.35-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于4.64pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格48T96T96T*596T*1096T*100
- 價格¥ 3268¥ 4668¥ 21006¥ 39678¥ 326760
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
實驗結果圖
標準曲線圖
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-92 | 85 |
| EDTA plasma(n=5) | 95-104 | 101 |
| heparin plasma(n=5) | 97-105 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-98% | 89-98% | 80-89% | 96-105% |
| EDTA plasma(n=5) | 83-91% | 90-103% | 98-105% | 92-101% |
| heparin plasma(n=5) | 84-93% | 78-103% | 86-101% | 95-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）25µL后，立即加入檢測溶液A25µL，
37°C孵育1小時；
3. 甩干，洗板3次；
4. 加檢測溶液B50µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物50µL，37°C孵育10－20分鐘；
7. 加終止液25µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質水平。將心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA225Mu02 | 心鈉肽(ANP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA225Mu01 | 心鈉肽(ANP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA225Mu02 | 心鈉肽(ANP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA225Mu01 | 心鈉肽(ANP)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA225Mu71 | 心鈉肽(ANP)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA225Mu | 心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA225Mu | 心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA225Mu | 心鈉肽(ANP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA225Mu | 心鈉肽(ANP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA225Mu11 | 心鈉肽(ANP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
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| Basic Research in Cardiology | Release of atrial natriuretic peptide precedes shedding of the endothelial glycocalyx equally in patients undergoing on- and off-pump coronary artery bypass surgery[PubMed: 21769675] |
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| Crit Care. | Hypervolemia increases release of atrial natriuretic peptide and shedding of the endothelial glycocalyx[Pubmed:25497357] |
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| Journal of Babylon University/Pure and Applied Sciences | Blood Pressure and Atrial Natriuretic Peptide (ANP) Levels in Patients with Autosomal Dominant Polycystic Kidney Disease.[papers:uobj_paper_2016_62613672.pdf] |
| PLoS One | Enhanced heart failure, mortality and renin activation in female mice with experimental dilated cardiomyopathy[pubmed:29240788] |
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| The Journal of Biological Chemistry | Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice[Pubmed:29929978] |
| Pediatric Cardiology | Determinants of Increased Aortic Diameters in Young Normotensive Patients With Turner Syndrome Without Structural Heart Disease[Pubmed:29392348] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure[Pubmed: 30230922] |
| Endokrynologia Polska | Endothelin-1 (ET-1), N-terminal fragment of proatrial natriuretic peptide (NTpro-ANP) and tumor necrosis factor alpha (TNF-α) in children with primary hypertension?…[Pubmed: 30359461] |
| International Journal of Peptide Research and Therapeutics | ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma[] |
| Van Veterinary Journal | Relationship between Natriuretic Peptide Levels with Heart and Kidney Parameters in Rats with Chronic Renal Failure.[] |
| Acta Pharmaceutica Sinica B | The poly (ADP-ribosyl) ation of BRD4 mediated by PARP1 promoted pathological cardiac hypertrophy[34094834] |
| Peptides | Alarin moderated myocardial hypertrophy via inhibiting cyclic adenosine monophosphate/protein kinase A signaling pathway to attenuate autophagy[34624429] |
| Peptides | Osteocrin alleviates cardiac hypertrophy via attenuating oxidative stress[Pubmed:35218795] |
